Utilize este identificador para referenciar este registo:
acessibilidade
http://hdl.handle.net/20.500.12207/679
Título: | Analysis of the expression of putative heat-stress related genes in relation to thermotolerance of cork oak |
Autor: | Correia, Barbara Rodriguez, José Luis Valledor, Luis Almeida, Tânia Santos, Conceição Canal, Maria Jesús Pinto, Glória |
Palavras-chave: | High temperature Plant performance Quercus suber Recovery |
Data: | 15-Mar-2014 |
Editora: | Elsevier |
Citação: | Correia, B., Rodriguez, J.L., Valledor, L., Almeida, T., Santos, C., Canal, M. J., & Pinto, G. (2014). Analysis of the expression of putative heat-stress related genes in relation to thermotolerance of cork oak. Journal of Plant Physiology, 171(6), 399–406. http://dx.doi.org/10.1016/j.jplph.2013.12.004 |
Resumo: | Cork oak (Quercus suber L.) is a research priority in the Mediterranean area and because of cork oaks’ dis-tribution these stands are experiencing daily stress. Based on projections of intensifying climate change and considering the key role of exploring the recovery abilities, cork oak seedlings were subjected to a cumulative temperature increase from 25 ◦C to 55 ◦C and subsequent recovery. CO2 assimilation rate, chlorophyll fluorescence, anthocyanins, proline and lipid peroxidation were used to evaluate plant per-formance, while the relative abundance of seven genes encoding for proteins of cork oak with a putative role in thermal/stress regulation (POX1, POX2, HSP10.4, HSP17a.22, CHS, MTL and RBC) was analyzed by qPCR (quantitative Polymerase Chain Reaction). A temperature change to 35 ◦C showed abundance alterations in the tested genes; at 45 ◦C, the molecular changes were associated with an antioxidant response, possibly modulated by anthocyanins. At 55 ◦C, HSP17a.22, MTL and proline accumulation were evident. After recovery, physiological balance was restored, whereas POX1, HSP10.4 and MTL abundances were suggested to be involved in increased thermotolerance. The data presented here are expected to pinpoint some pathways changes occurring during such stress and further recovery in this particular Mediterranean species. |
Arbitragem científica: | yes |
URI: | http://hdl.handle.net/20.500.12207/679 |
Versão do Editor: | http://dx.doi.org/10.1016/j.jplph.2013.12.004 |
Aparece nas coleções: | D-BIO - Artigos em revistas com peer review |
Ficheiros deste registo:
Não existem ficheiros associados a este registo.
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.