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http://hdl.handle.net/20.500.12207/6045
Title: | Purification of plant complex protein extracts in non-denaturing conditions by in-solution isoelectric focusing |
Authors: | Ferreira, Renata A. Martins-Dias, Susete |
Keywords: | Isoelectric focusing Plant extracts Plant leaves Plant proteins Poaceae |
Issue Date: | 15-Sep-2016 |
Publisher: | Elsevier |
Citation: | Ferreira, R. & Martins-Dias, S. (2016). Purification of plant complex protein extracts in non-denaturing conditions by in-solution isoelectric focusing. Analytical Biochemistry, 509, 100-103. http://dx.doi.org/10.1016/j.ab.2016.07.004 |
Abstract: | An alternative approach for plant complex protein extracts pre-purification by in-solution isoelectric focusing in non-denaturing conditions is presented. The separation of biologically active proteins, in narrow ranges of isoelectric point (pI) was obtained by a modified OFFGEL electrophoresis. Two different water-soluble protein extracts from Phragmites leaves were fractionated into 24 fractions within a 3-10 pI range at 10 °C in the absence of denaturing/reducing agents. One-dimensional electrophoretic analysis revealed different protein distribution patterns and the effective fractionation of both protein extracts. Peroxidase activity of each fraction confirmed that proteins remained active and pre-purification occurred. Biological triplicates assured the needed reproducibility. |
Peer reviewed: | yes |
URI: | https://hdl.handle.net/20.500.12207/6045 |
metadata.dc.identifier.doi: | https://dx.doi.org/10.1016/j.ab.2016.07.004 |
ISSN: | 0003-2697 |
Publisher version: | http://www.elsevier.com/wps/product/cws_home/622781/description |
Appears in Collections: | D-TCA - Artigos em revistas indexadas à WoS/Scopus |
Files in This Item:
File | Description | Size | Format | |
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Purification of plant complex protein extracts_PDFA.pdf | 419.77 kB | Adobe PDF | View/Open |
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