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http://hdl.handle.net/20.500.12207/6045
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DC Field | Value | Language |
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dc.contributor.author | Ferreira, Renata A. | - |
dc.contributor.author | Martins-Dias, Susete | - |
dc.date.accessioned | 2023-11-21T12:15:01Z | - |
dc.date.available | 2023-11-21T12:15:01Z | - |
dc.date.issued | 2016-09-15 | - |
dc.identifier.citation | Ferreira, R. & Martins-Dias, S. (2016). Purification of plant complex protein extracts in non-denaturing conditions by in-solution isoelectric focusing. Analytical Biochemistry, 509, 100-103. http://dx.doi.org/10.1016/j.ab.2016.07.004 | por |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.uri | https://hdl.handle.net/20.500.12207/6045 | - |
dc.description.abstract | An alternative approach for plant complex protein extracts pre-purification by in-solution isoelectric focusing in non-denaturing conditions is presented. The separation of biologically active proteins, in narrow ranges of isoelectric point (pI) was obtained by a modified OFFGEL electrophoresis. Two different water-soluble protein extracts from Phragmites leaves were fractionated into 24 fractions within a 3-10 pI range at 10 °C in the absence of denaturing/reducing agents. One-dimensional electrophoretic analysis revealed different protein distribution patterns and the effective fractionation of both protein extracts. Peroxidase activity of each fraction confirmed that proteins remained active and pre-purification occurred. Biological triplicates assured the needed reproducibility. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | por |
dc.subject | Isoelectric focusing | por |
dc.subject | Plant extracts | por |
dc.subject | Plant leaves | por |
dc.subject | Plant proteins | por |
dc.subject | Poaceae | por |
dc.title | Purification of plant complex protein extracts in non-denaturing conditions by in-solution isoelectric focusing | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.elsevier.com/wps/product/cws_home/622781/description | por |
degois.publication.firstPage | 100 | por |
degois.publication.lastPage | 103 | por |
degois.publication.title | Analytical Biochemistry | por |
degois.publication.volume | 509 | por |
dc.identifier.doi | https://dx.doi.org/10.1016/j.ab.2016.07.004 | por |
Appears in Collections: | D-TCA - Artigos em revistas indexadas à WoS/Scopus |
Files in This Item:
File | Description | Size | Format | |
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Purification of plant complex protein extracts_PDFA.pdf | 419.77 kB | Adobe PDF | View/Open |
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