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http://hdl.handle.net/20.500.12207/6073
Title: | Tribenuron-methyl metabolism and the rare Pro197Phe double mutation together with 2,4-D metabolism and reduced absorption can evolve in papaver rhoeas with multiple and cross herbicide resistance to ALS inhibitors and auxin mimics |
Authors: | Palma-Bautista, Candelario Portugal, João Vázquez-Garcia, José G. Osuna, Maria D. Torra, Joel Lozano-Juste, Jorge Gherekhloo, Javid De Prado, Rafael |
Keywords: | 2,4-D Cytochrome P450 enhanced metabolism Ligand docking Reduced transport Synthetic auxin herbicides Target site mutation |
Issue Date: | 11-Sep-2022 |
Publisher: | ELSEVIER |
Citation: | Palma-Bautista, C., Portugal, J., Vazquez-García, J. G., Osuna, M. D.,Torra, J., Lozano-Juste, J., Gherekhloo, J. & De Prado, R. (2022). Tribenuron-methyl metabolism and the rare Pro197Phe double mutation together with 2,4-D metabolism and reduced absorption can evolve in papaver rhoeas with multiple and cross herbicide resistance to ALS inhibitors and auxin mimics. Pesticide Biochemistry and Physiology, 188, 1-9. https://doi.org/10.1016/j.pestbp.2022.105226 |
Abstract: | Multiple resistance mechanisms to ALS inhibitors and auxin mimics in two Papaver rhoeas populations were investigated in wheat fields from Portugal. Dose-response trials, also with malathion (a cytochrome P450 inhibitor), cross-resistance patterns for ALS inhibitors and auxin mimics, alternative herbicides tests, 2,4-D and tribenuron-methyl absorption, translocation and metabolism experiments, together with ALS activity, gene sequencing and enzyme modelling and ligand docking were carried out. Results revealed two different resistant profiles: one population (R1) multiple resistant to tribenuron-methyl and 2,4-D, the second (R2) only resistant to 2,4-D. In R1, several target-site mutations in Pro197 and enhanced metabolism (cytochrome P450-mediated) were responsible of tribenuron-methyl resistance. For 2,4-D, reduced transport was observed in both populations, while cytochrome P450-mediated metabolism was also present in R1 population. Moreover, this is the first P. rhoeas population with enhanced tribenuron-methyl metabolism. This study reports the first case for P. rhoeas of the amino acid substitution Pro197Phe due to a double nucleotide change. This double mutation could cause reduced enzyme sensitivity to most ALS inhibitors according to protein modelling and ligand docking. In addition, this study reports a P. rhoeas population resistant to 2,4-D, apparently, with reduced transport as the sole resistance mechanism. |
Peer reviewed: | yes |
URI: | https://hdl.handle.net/20.500.12207/6073 |
metadata.dc.identifier.doi: | https://doi.org/10.1016/j.pestbp.2022.105226 |
ISSN: | 1095-9939 |
Publisher version: | https://www.sciencedirect.com/journal/pesticide-biochemistry-and-physiology |
Appears in Collections: | D-BIO - Artigos em revistas indexadas à WoS/Scopus |
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File | Description | Size | Format | |
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Tribenuron-methyl_JP.pdf | 1.62 MB | Adobe PDF | View/Open |
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