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http://hdl.handle.net/20.500.12207/6073
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DC Field | Value | Language |
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dc.contributor.author | Palma-Bautista, Candelario | - |
dc.contributor.author | Portugal, João | - |
dc.contributor.author | Vázquez-Garcia, José G. | - |
dc.contributor.author | Osuna, Maria D. | - |
dc.contributor.author | Torra, Joel | - |
dc.contributor.author | Lozano-Juste, Jorge | - |
dc.contributor.author | Gherekhloo, Javid | - |
dc.contributor.author | De Prado, Rafael | - |
dc.date.accessioned | 2023-12-06T11:39:12Z | - |
dc.date.available | 2023-12-06T11:39:12Z | - |
dc.date.issued | 2022-09-11 | - |
dc.identifier.citation | Palma-Bautista, C., Portugal, J., Vazquez-García, J. G., Osuna, M. D.,Torra, J., Lozano-Juste, J., Gherekhloo, J. & De Prado, R. (2022). Tribenuron-methyl metabolism and the rare Pro197Phe double mutation together with 2,4-D metabolism and reduced absorption can evolve in papaver rhoeas with multiple and cross herbicide resistance to ALS inhibitors and auxin mimics. Pesticide Biochemistry and Physiology, 188, 1-9. https://doi.org/10.1016/j.pestbp.2022.105226 | por |
dc.identifier.issn | 1095-9939 | - |
dc.identifier.uri | https://hdl.handle.net/20.500.12207/6073 | - |
dc.description.abstract | Multiple resistance mechanisms to ALS inhibitors and auxin mimics in two Papaver rhoeas populations were investigated in wheat fields from Portugal. Dose-response trials, also with malathion (a cytochrome P450 inhibitor), cross-resistance patterns for ALS inhibitors and auxin mimics, alternative herbicides tests, 2,4-D and tribenuron-methyl absorption, translocation and metabolism experiments, together with ALS activity, gene sequencing and enzyme modelling and ligand docking were carried out. Results revealed two different resistant profiles: one population (R1) multiple resistant to tribenuron-methyl and 2,4-D, the second (R2) only resistant to 2,4-D. In R1, several target-site mutations in Pro197 and enhanced metabolism (cytochrome P450-mediated) were responsible of tribenuron-methyl resistance. For 2,4-D, reduced transport was observed in both populations, while cytochrome P450-mediated metabolism was also present in R1 population. Moreover, this is the first P. rhoeas population with enhanced tribenuron-methyl metabolism. This study reports the first case for P. rhoeas of the amino acid substitution Pro197Phe due to a double nucleotide change. This double mutation could cause reduced enzyme sensitivity to most ALS inhibitors according to protein modelling and ligand docking. In addition, this study reports a P. rhoeas population resistant to 2,4-D, apparently, with reduced transport as the sole resistance mechanism. | por |
dc.language.iso | eng | por |
dc.publisher | ELSEVIER | por |
dc.relation | This work has been supported by the Asociacion de Agroquimicos y Medio Ambiente, Spain. Joel Torra acknowledges support from the Spanish Ministry of Science, Innovation, and Universities (grant Ramon y Cajal RYC2018–023866-I) and by the Spanish State Research Agency, Spain (AEI) and the European Regional Development Fund, EU (ERDF) through the projects AGL2017-83325-C4-2-R and PID2020-113229RB- C42. The field surveys made in Portugal were supported by Foundation for Science and Technology through the project UIDB/05064/2020 (VALORIZA). Jorge Lozano-Juste group is funded by grants RYC2020- 029097-I and PID2021-128826OA-I00 from Ministerio de Ciencia e Innovaci´ on (MCIN, Spain), AEI and the ERDF. | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | por |
dc.subject | 2,4-D | por |
dc.subject | Cytochrome P450 enhanced metabolism | por |
dc.subject | Ligand docking | por |
dc.subject | Reduced transport | por |
dc.subject | Synthetic auxin herbicides | por |
dc.subject | Target site mutation | por |
dc.title | Tribenuron-methyl metabolism and the rare Pro197Phe double mutation together with 2,4-D metabolism and reduced absorption can evolve in papaver rhoeas with multiple and cross herbicide resistance to ALS inhibitors and auxin mimics | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.sciencedirect.com/journal/pesticide-biochemistry-and-physiology | por |
degois.publication.firstPage | 1 | por |
degois.publication.lastPage | 9 | por |
degois.publication.title | Pesticide Biochemistry and Physiology | por |
degois.publication.volume | 188 | por |
dc.identifier.doi | https://doi.org/10.1016/j.pestbp.2022.105226 | por |
Appears in Collections: | D-BIO - Artigos em revistas indexadas à WoS/Scopus |
Files in This Item:
File | Description | Size | Format | |
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Tribenuron-methyl_JP.pdf | 1.62 MB | Adobe PDF | View/Open |
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