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http://hdl.handle.net/20.500.12207/6060
Title: | Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Carduus acanthoides |
Authors: | Palma-Bautista, Candelario Belluccini, Pablo Vázquez-Garcia, José G. Alcántara-de la Cruz, Ricardo Barro, Francisco Portugal, João De Prado, Rafael |
Keywords: | Auxinic herbicides Cytochrome P450 Herbicide metabolism Plumeless thistle Pro-106-Ser 5-enolpyruvylshikimate-3-phosphate synthase |
Issue Date: | 11-Feb-2023 |
Publisher: | Elsevier |
Citation: | Palma-Bautista, C., Belluccini, P., Vazquez-García, J., Alcantara-de la Cruz, R., Barro, F., Portugal, J., & De Prado, R. (2023). Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Cardus acanthoides. Pesticide Biochemistry and Physiology, 191, 1-6. https://doi.org/10.1016/j.pestbp.2023.105371 |
Abstract: | Carduus acanthoides L. is mainly a range-land weed, but in the 2010s has begun to invade GM crop production systems in C´ ordoba (Argentina), where glyphosate and 2,4-D have been commonly applied. In 2020, C. acanthoides was found with multiple resistance to these two herbicides. In this study, the mechanisms that confer multiple resistance to glyphosate and 2,4-D, were characterized in one resistant (R) population of C. acanthoides in comparison to a susceptible (S) population. No differences in 14C-herbicide absorption and translocation were observed between R and S populations. In addition, 14C-glyphosate was well translocated to the shoots (~30%) and roots (~16%) in both R and S plants, while most of 14C-2,4-D remained restricted in the treated leaf. Glyphosate metabolism did not contribute to resistance of the R population; however, as corroborated by malathion pretreatment, the mechanism of resistance to 2,4-D was enhanced metabolism (63% of the herbicide) mediated by cytochrome P450 (Cyt-P450). No differences were found in baseline EPSPS activity, copy number, and/or gene expression between the R and S populations, but a Pro-106-Ser mutation in EPSPS was present in the R population. Multiple resistances in the R population of C. acanthoides from Argentina were governed by target site resistance (a Pro-106 mutation for glyphosate) and non-target site resistance (Cyt-P450- based metabolic resistance for 2,4-D) mechanisms. This is the first case of resistance to glyphosate and 2,4-D confirmed for this weed in the world. |
Peer reviewed: | yes |
URI: | https://hdl.handle.net/20.500.12207/6060 |
metadata.dc.identifier.doi: | https://doi.org/10.1016/j.pestbp.2023.105371 |
ISSN: | 1095-9939 |
Publisher version: | https://www.sciencedirect.com/journal/pesticide-biochemistry-and-physiology |
Appears in Collections: | D-BIO - Artigos em revistas indexadas à WoS/Scopus |
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