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http://hdl.handle.net/20.500.12207/6060
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DC Field | Value | Language |
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dc.contributor.author | Palma-Bautista, Candelario | - |
dc.contributor.author | Belluccini, Pablo | - |
dc.contributor.author | Vázquez-Garcia, José G. | - |
dc.contributor.author | Alcántara-de la Cruz, Ricardo | - |
dc.contributor.author | Barro, Francisco | - |
dc.contributor.author | Portugal, João | - |
dc.contributor.author | De Prado, Rafael | - |
dc.date.accessioned | 2023-11-30T10:20:22Z | - |
dc.date.available | 2023-11-30T10:20:22Z | - |
dc.date.issued | 2023-02-11 | - |
dc.identifier.citation | Palma-Bautista, C., Belluccini, P., Vazquez-García, J., Alcantara-de la Cruz, R., Barro, F., Portugal, J., & De Prado, R. (2023). Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Cardus acanthoides. Pesticide Biochemistry and Physiology, 191, 1-6. https://doi.org/10.1016/j.pestbp.2023.105371 | por |
dc.identifier.issn | 1095-9939 | - |
dc.identifier.uri | https://hdl.handle.net/20.500.12207/6060 | - |
dc.description.abstract | Carduus acanthoides L. is mainly a range-land weed, but in the 2010s has begun to invade GM crop production systems in C´ ordoba (Argentina), where glyphosate and 2,4-D have been commonly applied. In 2020, C. acanthoides was found with multiple resistance to these two herbicides. In this study, the mechanisms that confer multiple resistance to glyphosate and 2,4-D, were characterized in one resistant (R) population of C. acanthoides in comparison to a susceptible (S) population. No differences in 14C-herbicide absorption and translocation were observed between R and S populations. In addition, 14C-glyphosate was well translocated to the shoots (~30%) and roots (~16%) in both R and S plants, while most of 14C-2,4-D remained restricted in the treated leaf. Glyphosate metabolism did not contribute to resistance of the R population; however, as corroborated by malathion pretreatment, the mechanism of resistance to 2,4-D was enhanced metabolism (63% of the herbicide) mediated by cytochrome P450 (Cyt-P450). No differences were found in baseline EPSPS activity, copy number, and/or gene expression between the R and S populations, but a Pro-106-Ser mutation in EPSPS was present in the R population. Multiple resistances in the R population of C. acanthoides from Argentina were governed by target site resistance (a Pro-106 mutation for glyphosate) and non-target site resistance (Cyt-P450- based metabolic resistance for 2,4-D) mechanisms. This is the first case of resistance to glyphosate and 2,4-D confirmed for this weed in the world. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier | por |
dc.relation | This work was funded by the Asociaci´ on de Agroquímicos y Medioambiente, Spainn and the Estaci´ on Experimental Agropecuaria Marcos Ju´arez, Argentina. | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | por |
dc.subject | Auxinic herbicides | por |
dc.subject | Cytochrome P450 | por |
dc.subject | Herbicide metabolism | por |
dc.subject | Plumeless thistle | por |
dc.subject | Pro-106-Ser | por |
dc.subject | 5-enolpyruvylshikimate-3-phosphate synthase | por |
dc.title | Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Carduus acanthoides | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.sciencedirect.com/journal/pesticide-biochemistry-and-physiology | por |
degois.publication.firstPage | 1 | por |
degois.publication.lastPage | 6 | por |
degois.publication.title | Pesticide Biochemistry and Physiology | por |
degois.publication.volume | 191 | por |
dc.identifier.doi | https://doi.org/10.1016/j.pestbp.2023.105371 | por |
Appears in Collections: | D-BIO - Artigos em revistas indexadas à WoS/Scopus |
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Target-site and non-target-site_JP_2023.pdf | 727.46 kB | Adobe PDF | View/Open |
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