Skip navigation
Please use this identifier to cite or link to this item:

Title: Molecular screening of ovine mastitis in different breeds
Authors: Guerreiro, O.
Velez, Z.
Alvarenga, N.
Matos, C.
Duarte, M.
Keywords: Subclinical mastitis
Sheep milk
DNA extraction
PCR-based screening method
Issue Date: Feb-2013
Citation: Guerreiro, O., Velez, Z., Alvarenga, N., Matos, C., Duarte, M. (2013). Molecular screening of ovine mastitis in different breeds. Journal of Dairy Science, 96 (2), 752–760.
Abstract: Clinical and subclinical mastitis directly affect mammary gland function and have a great economic impact on the sheep and goat dairy industries. The present study explores molecular diagnosis of ovine subclinical mastitis as a faster and more precise screening method compared with microbiology and biochemical techniques to assess the molecular and chemical properties of raw milk samples from healthy animals from 3 breeds of sheep raised in Portugal. Based on 16S ribosomal RNA screening by PCR, milk samples from all sheep were categorized as contaminated (n = 123) or noncontaminated (n = 104). For contaminated milk, different specific primers were used for pathogen identification (Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, and Streptococcus uberis). Streptococcus agalactiae was identified as the most frequent agent. We further assessed whether contaminated versus noncontaminated samples were chemically different in terms of fat, protein, lactose, pH, and solids-not-fat. This molecular screening method allowed rapid and efficient identification of contaminated raw sheep milk, including pathogen identification, before significant alterations in milk chemical properties could be detected. This methodology may lead to a specific and efficient animal treatment and consequently less expensive flock management.
Peer reviewed: yes
Publisher version:
Appears in Collections:D-TCA - Artigos em revistas com peer review

Files in This Item:
There are no files associated with this item.

FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Currículo DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.